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KMID : 0545120090190060566
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Journal of Microbiology and Biotechnology
2009 Volume.19 No. 6 p.566 ~ p.572
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Study on Immunostimulating Activity of Macrophage Treated with Purified Polysaccharides from Liquid Culture and Fruiting Body of Lentinus edodes
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Lee Hee-Hwan
Lee Jong-Seok
Cho Jae-Youl
Kim Young-Eon
Hong Eock-Kee
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Abstract
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Lentinus edodes is a well-known edible and medicinal mushroom used in Oriental cultures. Recently, L. edodes has attracted a lot of attention owing to its antifungal activity, antibacterial activity, antiviral activity, hepatoprotective effect, antitumor activities, and immunomodulatory and cytotoxic effects. In this study, the water-soluble crude polysaccharides, CPF and CPB, which were obtained from the fruiting body and culture cell-free broth of L. edodes by hot-water extraction and ethanol precipitation, were fractionated by DEAE cellulose and Sepharose CL-6B column chromatography, resulting in six polysaccharide fractions, CPFN-G-I, CPFN-G-II, CPFN-G-III, CPFA-G, CPBN-G, and CPBA-G. Among these fractions, CPFN-GI, CPBN-G, and CPBA-G were shown to stimulate the functional activation of macrophages including NO production cytokine expression and phagocytosis.
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KEYWORD
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Lentinus edodes, immunostimulating polysaccharide, mushroom-derived β-glucan, macrophage activation, phagocytosis
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